Optimization of regeneration using different plant growth regulator in Pomegranate (Punica granatum L.) cv.'Bhagwa' cultivar

A micropropagation technique is used for the pomegranate variety \u0027Bhagwa\u0027 with nodal segments as explants in present research. In current study, disease free culture were developed using a mixture of different concentrations sterilizing agents like carbendazim-50 per cent, cefotaxime, kanamycin, ketokenazol, and mercuric chloride (HgCl2). In addition to this, the phenolic exudates interfere with explant growth during In vitro condition. Consequently, the best approached proven to be transfer of explants at a regular interval of 24 h is best approach for phenolic exudates. Nodal segments were cultured on full strength Murashige and Skoog (MS) for shoot induction. The media was prepared supplemented with 6-benzylaminopurine (BAP), 0 to 2.5 mgl−1 and Kinetin (KIN), 0 to 1.0 mgl−1 produced maximum number of shoots (2.38±0.02) among all the treatments tested for shoot induction. Moreover, BAP with NAA proved to be best combination with adenine sulphate (30 mgl-1) and silver nitrate (1 mgl-1) for multiple shoot development gives produced more than 12 shoots (13.26±0.26) in 21 days intervals. Maximum number of root length and number of root recorded using ½ WPM with low concentration of IBA. The plantlets with well-formed root systems were progressively acclimatized in the greenhouse using soil, cocopeat (3:1) mixture and then transferred. Moreover, scanning electron microscopy (SEM) studies were carried out to observe and differentiate stomata and morphology of in vitro Pomegranate (Punica granatum L.) cv.\u0027Bhagwa\u0027