Sequential production of ligninolytic, xylanolytic, and cellulolytic enzymes by Trametes hirsuta AA-017 under different biomass of Indonesian sorghum accessions-induced cultures

Abstract Biorefinery concept encourages agricultural sector for developing zero waste technology. Utilization of agricultural residues for producing valuable products has become an important strategy to be investigated. Sorghum (Sorghum bicolor L) biomass, as one of agricultural residues, is potentially of great significance as a lignocellulosic material for production of lignocellulolytic enzymessuch as Laccase (Lac), Manganese Peroxidase (MnP), and Lignin Peroxidase (LiP), Cellulases (Cel), and Xylanases (Xyl). In the present study, 13 Indonesian sorghum accessions (with different lignocellulosic characteristics) were investigated due to their potency as potential substrate for production ligninolytic, hemicellulolytic, and cellulolytic enzymes by Trametes hirsuta AA-017 which has ability to sequentially produce the enzymes. Three accessions (Samurai, 4183, Kawali) were found as most suitable materials for enzymes production by the fungus with highest enhancement were more than 8000 and 71-fold, respectively for Lac and MnP. Total lignin in the biomass has shown a positive correlation with Lac (Pearson coefficient= 0.630; P