Screening and formulation of culture conditions for pyruvate production by candida glabrata MCC 1800 from corn steep liquor

Eleven culture parameters were screened by the Plackett–Burman design for pyruvate production to develop a standard protocol for media formulation. In an incubator shaker, the screened optimal conditions for pyruvate production were: carbon source 24.642g/L, nitrogen source 11.89 g/L, KH2PO4 1.185 g/L, thiamine 13.302 µg/L, biotin 13.927 µg/L, CaCO3 43.432g/L, MgSO4•7H2O 0.346 g/l, inoculum age 3 days, inoculum volume 6% (v/v), agitation 220 rpm, incubation time 46.412 h and incubation temperature 34.582℃. Except CaCO3 all factors were significant and agitation was considered a dummy factor in the experiment.