Genetic Separation of Chalkiness by Hybrid Rice of Huanghuazhan and CS197

The present study focused on the segregation of the percentage of grains with chalkiness (PGWC), using Huanghuazhan as the female parent and CS197 as the male parent to construct the hybrid rice F-2 population. Molecular markers were used for genotype analysis among the extremely low and extremely high PGWC individuals from the F-2 population. The results revealed that the genotypes of 10 extremely low PGWC individuals were 80.00% and 3.33%, which is identical to Huanghuazhan and CS197, respectively. The heterozygotes accounted for 16.67%. On the contrary, the genotypes of 10 extremely high PGWC individuals were 37.78% and 32.22%, which is identical to Huanghuazhan and CS197, respectively. The heterozygotes accounted for 30.00%. The extremely low and extremely high PGWC individuals had high coincidence with the corresponding F-2:3 family lines in phenotype and genotype. Scanning electron microscopy (SEM) images showed that the shape and spacing of starch granules in the extreme low and high PGWC individuals corresponded to Huanghuazhan and CS197, respectively. Correlation analysis showed that the F-2 individuals showed a normal distribution trending towards Huanghuazhan. The broad heritability sense was 26.31%, and the narrow heritability sense was 17.21%. The results of cluster analysis showed that the range of PGWC variations of group III based on phenotype varied from 5.66% to 50.00% with an average of 25.46%, and group II results based on genotype varied from 14.34% to 60.67% with an average of 32.33%. Groups II and III shared 28 identical individuals.