Evaluation of Ag, Cu, Fe and MnO2 nanoparticle mixture effecton histomorphological state of internal organs and tissues in laying hens
UKRAINIAN JOURNAL OF ECOLOGY(2020)
Abstract
The article presents the results of an impact assessment of a mixture of nanoparticles (MNPs) of metals (Ag, Cu, Fe) and manganese oxide (MnO2) on the histomorphological state of internal organs and tissues of laying hens. The study of a subchronic effect of an experimental sample of MNPs on the organism of laying hens was conducted on a Hayrase Brown crossbreed (n=112), weighing 1.5-1.8 kg at the age of 365 days. Colloidal solutions of MNPs (group II - 0.3 mg/kg, group III - 4.0 mg/kg body weight) and a mixture of salts of a corresponding metals (group I - 0.3 mg/kg body weight) were daily administrated to experimental groups of poultry as feed compound additives during 30 days. Distilled water was added to the compound feed for a control group of hens. Euthanasia of chickens (n=7) was performed by using inhalation chloroform anesthesia on a 15th and 30th day after exposure as well as 7 and 15 days after a termination of the drug exposure. Internal organs were selected for histomorphological studies. It has been found that changes of the morphological structure of the liver and kidneys established in chickens under the influence of MNPs in a toxic dose and occurred on the 30th day after the start and on the 7th day after the termination of it's a exposure. There were a thickness of the walls and lesions of the epithelial cells of the bile duct, a desquamation of a part of epithelial cells, a presence of granular dystrophy of the epithelium of the urinary tubules with elements of necrosis and hemorrhages. This testifies to the presence of dystrophic changes in hepatocytes, bile ducts, bile passages and urinary tubules.
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Key words
nanoparticles,dose,laying hens,internal organs,histological examination
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