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Utilization Of Local Fertilizer And Spring Water Combination For Growth Medium Of Stanieria Hs-48 (Cyanobacteria) As A Biofuel Feedstock

4TH INTERNATIONAL TROPICAL RENEWABLE ENERGY CONFERENCE (I-TREC 2019)(2020)

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Abstract
The study of NPK fertilizer medium had been done and it can be used for microalgae growth including cyanobacteria. Stanieria has the potential as a biofuel feedstock because it contains lipid in the form of fatty acids. Strain HS-48 was isolated from Ciater hot spring located in West Java, Indonesia. This study aimed to determine the best concentration of local NPK fertilizer that good growth medium for Stanieria HS-48. Stanieria HS-48 was grown on the BBM as a control, Lab. Alam water, NPK 350 ppm, and NPK 1050 ppm. This study was conducted for 14 days with 2 repetitions in each treatment. The average cell density (cell/mL) of Stanieria HS-48 at peak in Lab. Alam water was 1.7375 x 10(5), BBM was 2.1125 x 10(5), NPK 350 ppm was 5.0375 x 10(5), and NPK 1050 ppm was 5,875 x 10(5). Meanwhile, the growth rate (r) at NPK 350 ppm was higher rather than NPK 1050 ppm. Based on macroscopic observations, the NPK 350 ppm had a green color and the NPK 1050 ppm had a yellowish color. The result of the study showed that NPK 350 ppm was a good growth medium for Stanieria HS-48.
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Key words
biofuel, cell density, Lab. Alam water, NPK fertilizer, Stanieria HS-48
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