Chemical constituents and antitumor activities of walnut green husk.

[Objectives] To identify chemical constituents and antitumor activities of walnut green husk. [Methods] The column chromatography and recrystallization were used to isolate 13 compounds from walnut green husk, and their structures were identified by modern spectroscopy. [Results] The 13 kinds of compounds isolated from walnut green husk were identified as 3, 5-dimethoxy-4-hydroxy benzene carbonic-7-O-β-D-pyranglucose（1）, myricananin F（2）, β-stitosterol（3）, oleanolic acid（4）, regiolone（5）, asiatic acid（6）, hederagenin（7）, β-daucosterin（8）,（4 R）-4,8-dihydroxy-α-tetralone-4-O-β-D-glucopyranoside（9）, 3β, 23-dihydroxy-urs-12-en-28-oic acid（10）, ursolic acid（11）, syringc acid（12）, and Juglanoside B（13）. Among these, compounds 1, 2, 6, 7, 9, and 10 were all isolated from this genus of plant for the first time. [Conclusions] Through screening for antitumor activity in vitro, it was found that compound 4 showed strong growth inhibitory activity against the proliferation of human hepatoma cells（HepG-2）, and compound 9 had certain growth inhibitory activity against the proliferation of human breast cancer cells（MCF-7）.