Collagen 1 Fiber Content Can Predict For Recurrence In Stage 1 Non-Small Cell Lung Cancer

Introduction: Non-small cell lung carcinoma (NSCLC) comprises 85% of all lung cancers. Since most stage 1 NSCLC can be cured by surgery alone, these patients generally do not receive further cancer treatment. However, 20-30% of them will have their lung cancer progressed and died from it. There is a major unmet need to early identify those individuals who are likely to recur so that they can benefit from current effective cancer treatments. Collagen 1 (Col1) fibers are a major structural component of the extracellular matrix (ECM) of tumors. While increased Col1 fibers have been associated with increased metastasis in breast cancer, the role of Col1 in NSCLC and its relationship to recurrence has not been previously examined. Method: We performed a blinded second harmonic generation (SHG) microscopy study on surgically resected tumor histology tissue sections obtained from twelve patients with non-recurrent stage 1 NSCLC and fourteen patients with recurrent stage 1 NSCLC from The National Lung Screening Trial (NLST) project conducted by the National Institute of Health (NIH). Col1 fibers were detected with SHG microscopy that detects an intrinsic signal derived from the noncentrosymmetric molecular structure of Col1 fibers. SHG microscopy of tumor regions was performed on the HE 2020 Apr 27-28 and Jun 22-24. Philadelphia (PA): AACR; Cancer Res 2020;80(16 Suppl):Abstract nr 4299.