Study of Anti-pathogenic Herbal materials for the Eco-friendly drugs in Aquaculture

This study is examination about herb medicines that counters the effects of a fish pathogens, and is for eco-pharmaceutical development in aquaculture industry. 13 kinds of herb medicines were selected, and the hot water extracts and ethanol extracts were tested for antibacterial, antiparasitic, antifungal and antiviral effects. Minimal inhibitory concentration (MIC) of 13 kinds of candidate herb medicines were measured, above all, hot water and ethanol extracts of In-jin (1/10 and 1/10² dilution) showed antibacterial activities against 14 kinds of fish pathogenic bacteria. Hot water extract and ethanol extract of Dang-gwi showed antiparasitic and antifungal activities, and the extract had strong antifungal activity especially (~1/10³ dilution). In antiviral activity against VHSV (Viral hemorrhagic septicemia virus), hot-water and ethanol extract of In-jin and Dang-gwi were effective down to 1/10³ dilution. From these results, In-jin and Dang-gwi were selected as effective herbs among 13 kinds of herb medicines and further study on the synergy effect using combination with in-jin and dang-gwi against fish pathogens will be applied to fish immune response.