Abstract 3872: Spatial and multi-omics characterization of the tumor microenvironment in colorectal cancer

Background: Colorectal cancer is a heterogeneous disease, harboring tremendous differences in genetic mutations, gene expression pathways, and the tumor microenvironment. While there has been recent progress in understanding immune infiltration patterns in colorectal cancer, the majority of patients are thus far largely refractory to immunotherapy, and the underlying mechanism remains poorly understood. Here, we present a large-scale multi-omics analyses on 161 colorectal cancer tumors. More specifically, we aim to characterize the spatial and phenotypic profiles as well as the relationships between the different cell populations in the tumor microenvironment using multiplex immunohistochemistry. Methods: Tissue microarrays were constructed, comprising the tumor core, close-to-edge, tumor edge, and adjacent normal tissues for each tumor. HE 2020 Apr 27-28 and Jun 22-24. Philadelphia (PA): AACR; Cancer Res 2020;80(16 Suppl):Abstract nr 3872.