Optimization of Protoplast Formation, Regeneration, and Viability in <i>Sorangium cellulosum</i>

Sorangium cellulosum can product many secondary metabolites that is unique structural and makes these microorganisms highly attractive for drug development, especially epothilone, on cancer cells a cytotoxic macrolide which is naturally produced by Soxhlet cellulose that have the action of microtubule stabilization, is a promising anticancer drug. In this research, the factors affecting the regeneration and preparation of the protoplast of Sorangium cellulosum were discussed, those were regeneration media, enzymes and osmotic stabilizers. This study provide the distruction for improving the production of epothilone through genome shuffling, mutation, fusion and transformation.