Pharmacodynamics And Pharmacokinetics Behaviour Of Insulin From Pegylated-Mucin Microparticles Coated With Ph Sensitive Polymer: Preparation And Characterization

The aim of this study was to develop a mucin-PEG based microparticles for oral delivery system for insulin for diabetes treatment. Mucin-PEG microparticles were prepared using emulsion technique. These microparticles were coated with Hydroxyl propyl methylcellulose acetate succinate (HPMCAS) using solvent evaporation method. The effect of polymer ratios and coating were investigated in terms of size, surface morphology, entrapment efficiency, micrometrics properties, in vitro release and in vivo studies. The size of insulin-loaded MPs is> 80 mu m and exhibited rough surface with irregular shape. The maximum and minimum encapsulation efficiency were 89.10 and 75.15 %, respectively. In vitro release study in pH 1.2 revealed insignificantly low (6 %) (p< 0.05) as compared to the significantly high (85 %) released obtained in pH of 7.4 for formulation combined PEG/mucin ratios (batches B1, B2 and B3). Furthermore, in vivo experiments in Wistar Albino rat model demonstrate that insulin-loaded MPs effectively reduced blood glucose levels over a period of 10 h after oral administration. Therefore, these findings clearly suggest that the HPMCP-coated PEG-mucin microparticles offer an interesting mode of insulin delivery orally for the treatment of diabetes.