Combined genotype and fatty-acid analysis of single small field pennycress (Thlaspi arvense) seeds increases the throughput for functional genomics and mutant line selection

•Single seed fatty-acid analysis increases the throughput of functional genomics.•Characterization of mutant alleles of Ta FAE1, Ta FAD2 and Ta ROD1.•Additive gene interactions create oil profiles with high oleic and super high oleic traits.•Molecular markers for enhanced oil traits in pennycress.