Viral detection in mNGS libraries.

A positive control consisting of 8 viruses and chlamydia trachomatis spiked into normal human plasma each at log 4.0 copies/ml was included with samples in three separate extractions, library preps and sequencing runs of HCV positive samples. Reads were taxonomically assigned by the SURPI pipeline. The top histogram represents the genome coverages and the bottom histograms represent reads per million for each pathogen. (PDF)