Efficient Shoot Organogenesis Using Leaf Disc And Nodal Explants Of Passion Fruit (Passiflora Edulis Sims) And Genetic Fidelity Assessment Using Sequence-Related Amplified Polymorphism (Srap) Markers

Passion fruit (Passiflora edulis(Sims)) is currently ranked third among fruit exports from Kenya and has great potential since the demand for both fresh fruit and processed juice is on a continuous increase. Passion fruit production in Kenya is constrained by a lack of healthy, clean planting material, poor seed viability, and low germination rates. To address this, the present study reports anin vitroplant regeneration protocol for passion fruit using leaf disc and nodal explants and genetic fidelity analysis of the regenerated plants. The highest number of shoot regeneration was obtained on Murashige and Skoog (MS) medium supplemented with 2 mg center dot L-1 6-Benzyl amino purine (BAP) (shoot induction medium). The multiplication of shoots was optimum in MS medium supplemented with 3 mg center dot L-1 BAP. To eliminate the requirement of an additional step ofin vitrorooting, exogenous application of putrescine induced the formation and development of roots on nodal explants. Genetic fidelity analysis of thein vitroregenerated and macropropagated plants with that of the mother plant was carried out by sequence-related amplified polymorphism (SRAP) markers, and monomorphic banding profile for 80% of the regenerants confirmed the genetic uniformity of thein vitroregenerated and macropropagated plants. Thein vitroregeneration system developed can be utilized for mass clonal propagation for the economic commercial exploitation of this important tropical fruit.