THE ANALYSIS OF ASSOCIATIONS OF HELICOBACTER PYLORI BABA GENE IN PATIENTS WITH CLINICAL OUTCOMES OF GASTRODUODENAL DISEASES IN YAKUTIA

Introduction: Helicobacter pylori has several of the most characteristic adhesins that have the property of a targeted effect on epithelial cells of human stomach, among which the babA gene is the most studied and exists in several variants - babA1, babA2. The BabA protein is high-affinity and binds to mono or difucosylated blood group antigens and each can be modified into blood group A, B, or 0 and expressed on epithelial cells of the stomach. The clinical outcomes of gastroduodenal diseases, depending on the babA variants of Helicobacter pylori circulated in Yakutia, has not been previously studied. The aim of this work is to analyze of associations of Helicobacter pylori babA gene in patients with clinical outcomes of gastroduodenal diseases in Yakutia. Materials and methods: Gastric biopsy specimens were obtained from 322 patients. According to the results of histological analysis, 188 patients had the presence of Helicobacter pylori and divided into two groups: chronic gastritis and chronic gastritis with erosions and ulcers. Results: Chronic gastritis was established in 96 samples (51,1%), and the diagnosis of chronic gastritis with erosions and ulcers was established in 92 samples (48,9%). Helicobacter pylori babA2 gene variant were identified in 65 samples (34,5%), and babA1 in 123 samples (65,4%). In the male patients the frequency of the babA2 gene was almost two times higher (69,6%) than in the female patients (38,3%) (p<0,001). In contrast, in female patients more common was the babA1 gene (61,6%) than in male patients (30,3%) (p<0,001). It was found that the babA2 gene variant was significantly more common in samples of patients with chronic gastritis associated with erosions and ulcers of stomach and duodenum (43,4%) than in patients with chronic gastritis (26,0%) (p<0,05). Patients diagnosed with chronic gastritis had more often the babA1 gene variant (73,9%), than patients with erosive gastritis (56,5%) (p<0,05). In comparing group of patients with different degrees of inflammation there were no statistically significant difference in the activity of inflammation with the presence of the babA gene, but there was a slight statistical difference with the second degree of dissemination which had the babA1 gene variant. Conclusion: We showed relationship between babA2 gene of Helicobacter pylori and more severe clinical outcomes (erosions and ulcers) in patients with gastroduodenal diseases in Yakutia. Obtained result confirms previously known about data babA2 which are more virulent and pathogenic than babA1 gene of Helicobacter pylori. The data about babA2 gene was more frequent in male patients and it may be an additional risk factor for more severe gastroduodenal diseases.