Growth of Arabidopsis thaliana in rhizobox culture system evaluated through the lens of root microbiome

Aims The present study provides an insight on physiological parameters and root-associated microbiome of two complementary Arabidopsis thaliana culture systems suitable for physiological studies. Methods A. thaliana plants were grown in rhizobox and in classic pot culture. An analysis of plant growth parameters along with the characterization of the bacterial communities of the compost and the rhizosphere, rhizoplane and endosphere compartments were performed. Results A. thaliana plants grown in rhizobox exhibited a plant habitus similar to those grown in pots during the first month, but with a delayed leaf initiation and slower rate of growth over the experiment. No nutrient deficiency symptoms were observed in these plants. The rhizobox design permits the migration of bacteria from compost to plant roots and allowed root-bacteria interactions to be established as in traditional pot culture. Some differences into the composition of the rhizobiome were highlighted between the two culture systems. Nevertheless, key bacterial taxa that help to ensure plant growth and health colonized the rhizo- and endosphere compartments in both systems. Conclusions This study gives some clues for future research programs and selection of efficient root systems with the use of such specific culture devices.