Bioconversion, Purification and In Vitro Antibacterial Activity of Platenomycin A1

Platenomycin A1 is one type of 16-membered macrolide antibiotics, which had been shown to inhibit the Gram-positive bacteria growth. In this study, the bioconversion of platenomycin A1 from midecamycin in Streptomyces thermotolerans was investigated. With optimized growth conditions, platenomycin A1 production reached to 5.654 g/L. In addition, the platenomycin A1 separation and purification procedures were developed based on pH adjustment and ODS reversed phase chromatography refinement, which leaded to a purity up to 97.74%. Minimum inhibitory concentrations (MIC) of purified platenomycin A1 against representive gram-positive and gram-negative bacteria strains were also determined, which displayed active inhibition against Staphyloccocus aureus (ATCC29213), Streptococcus pneumonia (ATCC49619), septic Streptococcus (CVCC593) and porcine Actinobacillus pleuropneumoniae (CVCC260).