Baicalin Suppress Nasopharyngeal Carcinoma Biological Activity Via Regulation miRNA-21

The aim of this study was to evaluate the effects and mechanism of baicalin in nasopharyngeal carcinoma suppression by in vitro study, collecting the adjacent and cancer tissues of nasopharyngeal carcinoma patients including I-II stage and III-IV stage and measured the miRNA-21 expression by ISH assay. The CNE-2Z cell were divided into 4 groups (NC, baicalin, baicalin + BL and baicalin + miRNA). Measuring cell proliferation by CCK-8 assay, cell apoptosis by flow cytometer, invasion cell number by transwell and wound healing rate by wound healing assay. Meanwhile, The PTEN, PI3K and AKT proteins expressions were measured by WB assay. The PTEN nuclear was evaluated by immunofluorescence. Compared with adjacent normal tissues, the miRNA-21 expression was significantly suppressed in nasopharyngeal carcinoma with stage increasing (P < 0.01 or P < 0.001, respectively). With baicalin supplement, the cell proliferation, invasion cell number and wound healing rate were significantly down-regulation and cell apoptosis was significantly up-regulation (P < 0.001, respectively). And the PI3K and AKT protein expressions were significantly suppressed and PTEN protein was increased in baicalin treated (P < 0.001, respectively). By Immunofluorescence, the PTEN nuclear was up-regulation in baicalin group. Baicalin could suppress nasopharyngeal carcinoma biological activities via regulation PTEN/PI3K/AKT by suppressing miRNA-21.