Development and Validation of High-Performance Liquid Chromatographic Method for Quantitative Estimation of Glycyrrhetinic Acid in Microemulsion

The aim of this study was to develop a simple, accurate, precise, and reproducible reverse-phase high-performance liquid chromatography method for the determination of glycyrrhetinic acid in microemulsion. The chromatographic separation was achieved on a chromatographic column HiQ Sil C-8 (250 x 4.6 mm, 5 mu m), for separation at a flow rate of 1 mL/min using 10 mM KH2PO4 buffer: Acetonitrik (5:95 v/v) as mobile phase and detection at 250 mu. The retention time was found to be 3.6 min. Method was linear over the range of 5-30 mu g/mL with regression coefficient 0.9983. The developed method can be applied for routine quality control analysis of glycyrrhetinic acid in microemulsion formulation. The method was validated as per the guideline of International Conference on Harmonization (ICH, Q2 [R1]). Hence, attempts were made to develop a simple, rapid, precise, and accurate reverse-phase chromatographic method for estimation of glycyrrhetinic acid in microemulsion formulation.