Phytochemical and Antimicrobial Analysis of some Citrus Fruits Juices from Sindh Pakistan against Selected Pathogens

The main objective of this study was to analyze phyto-chemical constituents from fresh citrus fruits and perform antimicrobial activity by K.B Disc Diffusion method and to compare zone of inhibition with standard antimicrobial drug. The results of phytochemical and antimicrobial analysis were observed from the citrus plants including Citrus paradisi, Citrus reticulate, Citrus sinensis, Citrus aurantifolia, Citrus limon, Citrus aurantium and Citrus jambhiri. Presence of various macromolecules such as tannins, phenols, proteins, amino acids, glycosides, steroids, flavonoids, carbohydrates, alkaloids, cholesterol, terpenoids and saponins were observed during photochemical analysis. Antimicrobial analysis revealed the zone of inhibition which was observed for these citrus fruits against E. coli, S. paratyphi B, Shigella sonnei and V. cholerae. Antifungal activity was found more in lemons and limes as compared to oranges. It was concluded that the fresh citrus fruit juices of Sindh contain potent antimicrobial compounds and they can be used as alternative medicines especially against enteric pathogens.