Carbon Nanostructured Screen‐printed Electrodes Modified with CuO/Glucose Oxidase/Maltase/SiO2 Composite Film for Maltose Determination

A sensitive voltammetric method was developed to determine maltose in beverage products using a carbon nanostructured screen-printed electrode modified with CuO/glucose oxidase/maltase/SiO(2)biocomposite film. Adding CuO particles was done to possess catalytic activity toward hydrogen peroxide. Electrode modified by glucose oxidase and maltase shows a good response to maltose. A well-defined reduction peak was registered at the potential of -0.55 V (vs. Ag/AgCl) which intensity increases linearly with the concentration of maltose ranging from 0.01 to 0.1 mmol L-1. The calculated limit of detection was 0.005 mmol L-1. Tested on the beer samples, the developed CuO/glucose oxidase/maltase/SiO(2)biocomposite film covered carbon nanostructured screen-printed electrode is showed to be a prospective sensitive element of the third generation biosensor for maltose.