Exosomes Derived From Tendon Stem Cells Promote Cell Proliferation And Migration Through The Tgf Beta Signal Pathway

Tendon stem cells (TSCs) are a kind of progenitor cells found in tendon niches, which play a key role in the repair of tendon injuries. Exosomes that mediate cell communication are involved in physiological processes and various diseases, while the effect of exosomes derived from TSCs (TSC-exo) on TSCs is still unclear. The purpose of this study is to explore the effect of TSC-exo on TSCs. Analyzing the characteristics of TSC-exo, we found that the TSC-exo were enriched in a large amount of transforming growth factor beta (TGF beta) by western blotting. We also found that the TGF beta carried by TSC-exo can effectively accelerate the proliferation and migration of TSCs. We further found that TGF beta carried by TSC-exo can activate the TGF beta-Smad2/3 and the ERK1/2 signaling pathway in TSCs. Furthermore, matrix metalloenzyme 2 (MMP2), a downstream molecule of Smad2, is regulated by TGF beta carried by TSC-exo. Collectively, our findings provide molecular insights into TSC-exo and indicate that TSC-exo are a potential strategy for treating tendon injuries. (C) 2020 Elsevier Inc. All rights reserved.