Structural characterization and developability assessment of sustained release hydrogels for rapid implementation during preclinical studies

•Mesh size, modulus, injectability, viscosity, Tsol-gel are important structural parameters and developability constraints for hydrogels.•Mesh size (SAXS/Rheology) of hydrogels > size (RH) of lysozyme suggesting that obstruction by polymer network doesn't hinder diffusion.•~2x greater amount of lysozyme released from PLCL hydrogel as compared to PLGA, due to the higher viscosity of PLGA, increasing frictional drag on lysozyme and reducing its diffusivity.•Tsol-gel (25 - 32 °C) and injectability (injection force < 20N) confirmed that these hydrogels can be rapidly implemented during preclinical development.