A "Turn-On" And Proximity Ligation Assay Dependent Dna Tweezer For One-Step Amplified Fluorescent Detection Of Dna

A "turn-on" and proximity ligation assay dependent DNA tweezer was proposed for one-step amplified fluorescent detection of DNA. Target DNA can anneal with capture probe to form an entire long sequence. The formed long sequence can circularly open the hairpin, resulting the "turn-on" of DNA tweezers. A good linear relationship was shown from 40 pM to 20 nM with limit of detection of 10 pM. In addition, it has been successfully utilized to analysis DNA in human serum, representing a great and practical application future. (C) 2020 Elsevier B.V. All rights reserved.