EFFECT OF CARBOHYDRATE TYPE OVER THE MICROSPORE EMBRYOGENESIS AT BRASSICA OLERACEA L.

Microspore culture triggers the attention of specialists all over the world, due to its importance in obtaining double haploid plants, a true homozygous line that can used in breeding for the obtaining of F-1 hybrids, shortening the period in which they can be obtained with at least 6-7 years. The ability to change from the normal gametophytic development of pollen to a sporophytic one, finalized with haploid embryo formation and ultimately with double haploid plants by spontaneous or induced diploidization is conditioned by many intrinsic and extrinsic factors. Among the many factors that affect the viability and the capacity of microspores cultivated on liquid mediums in vitro to switch their developmental programs from gametophytic way to the sporohytic one, finalized with the obtaining of double haploid embryos and plants, the carbohydrates play a critical role. They act both as a source of energy for the growth and development of cells and as a osmoticum. To investigate the effects of different carbon sources on embryo induction in liquid cultivation system, microspores were cultured in NLNS medium containing 6, 9, or 12% (all w/v) of sucrose, fructose, glucose and maltose.