Membrane Drug Target Identification in Mycoplasma Pneumonia-A Subtractive Genomic Approach

The ease of use of genome sequences of pathogens has provided a remarkable quantity of information that can be used in identification of drug targets and vaccine production. The application of in silico subtractive genomics technology drastically shortens the time required for such purposes. Subtractive genomics approach enables the subtraction dataset between the host and pathogen. The aim of present work is to identify the Drug targets in Mycoplasma Pneumonia by subtractive genomic approach which helps to identify the homolog's and Non homologs between the host and pathogen. The availability of complete Proteome sequence information of Mycoplasma Pneumonia from NCBI-FTP site helps to perform the BLAST against human proteome database. The screening of these proteins using different database like essential genes (DEG), metabolic pathways KEGG helps to identify essential proteins which may be used as drug targets against Mycoplasma pneumonia. Prediction of these essential proteins of the outer membrane of the pathogen also could be potential vaccine targets