Invitro Antioxidant and Anti-salmonellal activities of StemBark extracts of Enantia chlorantha (Annonaceae)

The aim of the present study was to investigate the in vitro anti-salmonellal and antioxidant activities of the extracts of this plant against five Salmonella species. The anti-salmonellal activity was evaluated using broth microdilution method, whereas the evaluation of antioxidant properties, the quantitative determination of total phenols and flavonoids and phytochemical screening were performed by standard methods. This study showed that the Enantia chloranta extracts have a beneficial effect on all the tested pathogens (Salmonella) with minimum inhibitory concentrations (MICs) ranging from 64 to 512 mu g/mL. MeOH, 95% EtOH and 70% EtOH extract have the highest antimicrobial activity on of the tested microorganisms. MeOH and 95% EtOH extracts exhibited the strongest antioxidant activities (IC50<20 mu g/mL) whereas water macerated extract presented among all extracts the lowest antioxidant activity (IC50<20 mu g/mL). Phytochemical screening of extracts revealed the presence of flavonoids and phenols in all the extracts and alkaloids, anthocyanins, anthraquinons, saponins, triterpenes and cardiac glycosids depending on the used extracts. In vitro antioxidant and anti-salmonellal activities of Enantia chloranta extracts support not only the local use of this plant for the treatment of typhoid fever, but also its use as possible source in the development of new drugs.