Cancer-Related Mutations Are Not Enriched in Naive Human Pluripotent Stem Cells.
Cell stem cell(2020)
Abstract
Previous analysis of RNA sequencing (RNA-seq) data from human naive pluripotent stem cells reported multiple point "mutations" in cancer-related genes and implicated selective culture conditions. We observed, however, that those mutations were only present in co-cultures with mouse feeder cells. Inspection of reads containing the polymorphisms revealed complete identity to the mouse reference genome. After we filtered reads to remove sequences of mouse origin, the actual incidence of oncogenic polymorphisms arising in naive pluripotent stem cells is close to zero.
MoreTranslated text
Key words
pluripotent stem cell,single nucleotide polymorphism,SNP,naive pluripotency,sequencing informatics,cancer-related mutations,TP53,genetic integrity
AI Read Science
Must-Reading Tree
Example
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
Generate MRT to find the research sequence of this paper
Chat Paper
Summary is being generated by the instructions you defined