Effects of oral heat shock protein 60 on serum matrix metalloproteinase-9 in atherosclerosis rats

Objective to study the effect of oral heat shock protein 60(HSP60) on serum matrix metalloproteinase‐9 (MMP‐9) in atherosclerosis (AS) rats. Methods 40 healthy male SD rats were randomly divided into normal control group (group C), atherosclerosis intervention group (AS group), oral HSP60 group before building AS model (H1 group), oral HSP60 group after building AS model (H2 group), 10 rats in each group, weight 100 + 10 g. AS model was made by high fat feed. At 180 days of age, the rats were executed after obtaining the rats blood. MMP‐9 content in rats serum of each group were detected by enzyme‐linked immuno sorbent assay (ELISA). Results The MMP‐9 content in rats serum of group C, AS group, H1group and H2 group respectively are 0.43±0.15μg/mL,3.83±0.64μg/mL,0.91±0.20μg/mL,1.91±0.70μg/mL Conclusion The content of MMP‐9 in serum of atherosclerosis rats was higher than that of normal control group. Oral administration of HSP60 could reduce the content of MMP‐9 in serum, which had certain effect on maintaining the stability of fibrous plaque. The earlier oral administration of HSP60, the better the effect. Support or Funding Information This study was supported by the National Natural Science Foundation Youth Fund (30600637), China Postdoctral Foundation(2014M561207), the Shanxi Province Basic Research Program Youth Science and Technology Research Fund (2010021034‐4, 201601D011119), the Shanxi Scholarship Council of China (2011‐096 and Key Projects No. 4), Department of Human Resources and Social Security of Shanxi province, The science and technology research projects of health department of Shanxi Province(2016D011119), Science and technology development project of colleges of Shanxi Province, Innovation fund for college students in Shanxi Province, The Teaching Reform Program of Shanxi Higher Education, The Education Reform Project of Postgraduate Students in Shanxi Province. The Education Reform Project of Shanxi Medical University.