Rapid Identification of Peanut Hybrids with SSR-PCR

To fulfill high efficiency peanut hybrid identification, an SSR-PCR rapid detection system was established by simplifying the process of DNA template preparation. After that, true hybrids from six F-1 populations were identified by morphological observation and SSR-PCR. The result showed that true and pseudo peanut hybrids were difficultly distinguished by morphological observation when hybrid parents were similar in morphology whereas it was easy by SSR-PCR. In the six F1 populations, 38-56% of putative hybrids were identified as true hybrids by SSR-PCR. Hybrid rates of peanut might vary among different combinations even in reciprocal crosses.