A draft genome sequencing using next generation sequencing technology in black sesame (Sesamum indicum L.)

Sesame (Sesamum orientale L.) is one of the most ancient and important oil seed crop, also known as S. indicum L. The diploid chromosome number of sesame is 2n=26 and it is usually self-pollinated. Sesame belongs to the Pedaliaceae family. Sesame GT-10 (Black sesame) oil is good source of some phyto-nutrients. In present study, ABI SOLiD (TM) and Ion Torrent Whole Genome Sequencing (WGS) technologies were used to generate sesame draft genome. For the measurement of the genome size, flow cytometer (Accuri C6) was used, found approximately 375Mb. The total raw sequence generated by SOLiD genome analyzer was 61.07 Gb. PGM generated 1.8 Gb data. The de novo assembly (using SOLiD analyzer) yielded assembled reads of 2,962,553,296 and number of contings was 63,785,429. de novo assembly of PGM (combined data four runs) yielded assembled reads of 48,729,832 with number of contings count as 62178.5. In the assembly the N25, N50, N75 conting size was 737.75, 533.5 and 396.5 bp respectively. In Blast 2GO analysis, total 94752 sequence were functionally annotated out of which 94705 (99.95%) showed positive interpro. While 71160 (75.10%) got Blast hits, 73.32% (69474) and 29.59% (28044) sequence were mapped and annotated while in gene ontology, total 101098 GO IDs were found among which 27.61% (36201), 49.70% (65165) and 22.67% (29732) were grouped into biological process, cellular components and molecular function, respectively. Using BatchPrimer3 (V 2.0), total 406 SSRs were identified with Tm and GC % range of 62.23-50.15 and 66.11-33.00 respectively.