IRON NANOPARTICLES' EFFECTIVENESS FOR NEW ANTIANEMIC PREPARATIONS DEVELOPMENT

In spite of considerable quantity of antianemic preparations with different iron forms and additives, which are presented on modern pharmaceutical market, iron deficiency anemia is one of the most wide-spread pathological states as well as social problems in the world. Pregnant women, children and elderly persons are particularly vulnerable. The situation is complicated by the presence of some disadvantages and drug side effects of existing antianemic medicines. Development of new way for antianemic preparations is very perspective. Iron nanoparticles are possessed by high potential in this area. For the purpose of new effective antianemic preparations' creation the technology of colloidal-chemical synthesis for 40 nm spherical iron nanoparticles in water soliutions has been developed. Synthesized iron nanoparticles have been characterized as biosafe using in vitro and in vivo tests. For biosafety level estimation parameters of cytotoxicity, genotoxicity, mutagenicity and main biochemical markers have been used. LD50 of synthesized nanoparticles in per os dosing (BALB/c mouse line) is more than 5 g/kg. Biological activity of the iron nanoparticles as potential pharmacological substance with antianemic properties has been studied on the model of iron deficiency anemia with Wistar rats and BALB/c mice females using. It has been shown reliable increasing of main blood parameters such as hemoglobin level, iron concentration in blood serum, transferrin saturation percentage up to normal level of healthy animals comparatively with control within 10 days of experimental treatment course under the conditions of per oral as well as intravenous route of iron nanoparticles' introduction.