TEST OF PHTHALOCYANINES ANTIMICROBIAL ACTIVITY

The aim of this study is to demonstrate antimicrobial activity of selected positively charged water-soluble phthalocyanines. 12 phthalocyanine derivatives were used for this study. All the tested compounds were synthesized in Centre of Organic Chemistry (COC, Rybitvi, Czech republic). Water soluble phthalocyanine derivatives were prepared by peripherally substitution of the phthalocyanine skeleton with the aim to prepare cationic phthalocyanines with an ability to have a wide-spectrum antimicrobial effect. To determine the antimicrobial effects of phthalocyanines, bacterial strains Escherichia coli CCM 3988, Enterococcus faecalis CCM 4224, and Pseudomonas aeruginosa CCM 1960 were used. Phthalocyanine samples were tested in two concentrations -0.1 and 0.5 g/l. In a sterile vial, phthalocyanine solution with the bacteria was shaken under artificial light at room temperature. The suspension was sampled at 1st, 2nd and 3rd hour. Samples were subsequently inoculated on Petri dishes with nutrient medium. The control sample was prepared by the same procedure. After the cultivation of Petri dishes, accrued colonies were counted and the results were expressed in CFU/ml. Antimicrobial action of phthalocyanines has been demonstrated.