Optimizing the dosing regimens for intravascular administrations of oncolytic adenovirus

The objective of this study was to determine the impact of multiple-dose regimens on the activity of the final dose of an oncolytic adenovirus. Groups of tumor-bearing mice were dosed with the oncolytic adenovirus 01/PEME on each of 1, 2, 3, or 4 consecutive days prior to final treatment with a single dose of a different oncolytic adenovirus carrying the reporter gene, dl01/07-luc. Delivery and replication of the dl01/07-luc in the tumor and liver were determined by quantifying virus DNA copies by PCR and reporter gene activity by a luciferase enzymatic assay. Our results showed that there was a regimen-dependent effect after multiple prior intravenous administrations on the biological activity of dl01/07-luc.