CHANGES IN ERYTHROCYTE ELECTROKINETIC CHARACTERISTICS AFTER THEIR PRESERVATION

The effects of erythrocyte preservation on their electrophoretic mobility (EPM) and morphology were studied. Erythrocytes were isolated from the blood, collected in CPDA-1 preserving agent, in 30 donors. Electrophoretic mobility was studied under a microscope in a special box with Ag/AgCl electrodes. The morphology of erythrocytes was studied under a Primo Star microscope (Carl Zeiss, Germany). The microvesicles (MV) were isolated from erythrocyte suspension by ultracentrifugation at 100 000 g. The erythrocyte EPM reduced significantly after preservation. One of the causes of this reduction was MV formation, caused, among other things, by disk-spheric transformation of erythrocytes - marked anisocytosis and poikilocytosis. These processes were more rapid in pre-washed erythrocytes, despite their storage in the same preserving solution. More intense and rapid morphological changes in erythrocytes were associated with greater reduction of their EPM and formation of MV.