Prognostic Value of Cyclin E and Cyclin Dependent Kinase Inhibitor (P27) Gene Expression in Non - Hodgkin's Lymphoma

The eukaryotic cell cycle is controlled by protein kinase complexes composed of cyclins and cyclin dependent Kinases (Cdks). The activity of Cdks is regulated by binding of positive effectors, the cyclins, and by association-dissociation of inhibitory subunits, designated cyclin dependent kinase inhibitors (CKIs). Cyclins, Cdks, and CKIs are frequently altered in human cancer. P27 is a CKI that regulates progression from G1 into S phase and appears to play a role in both cell growth and differentiation. Cyclin E, in conjunction with its kinase partner Cdk2, regulates many aspects of cell division. Many human cancers express high levels of cyclin E, and this is thought to directly contribute to cell transformation and tumor aggressiveness. The aim of this study was to study the prognostic value of p27 and cyclin E protein expression levels in relation to the staging of NHL, laboratory data, clinical manifestations and to predict patient's survival. The patients were subjected to full work-up for diagnosis of NHL and Western blot analysis for detection of p27 and cyclin E protein expression. 40 newly diagnosed patients suffering from non-Hodgkin's lymphoma (NHL) in different stages and twenty healthy subjects were the subject matter of this study. Our results showed over expression of cyclin E and down regulation of p27, which was significantly associated with advanced staging of the disease. There was a positive correlation between age and over expression of cyclin E and inverse correlation with p27 expression. Over expression of cyclin E and down regulation of p27 were significantly associated with laboratory and clinical findings, delayed remission, increased relapse and increased death rate. Conclusion: p27 and cyclin E expression are significant, independent prognostic factors and reliable molecular markers in predicting recurrence and selection of patients for adjuvant therapy in malignant lymphoma. [Manal A. Eid; Basma M. Elgamal; Ghada M. Ezat; Eman A. Amer and Hoda A. Salem. Prognostic Value of Cyclin E and Cyclin Dependent Kinase Inhibitor (P27) Gene Expression in Non - Hodgkin's Lymphoma. Life Sci J 2012; 9(2): 692-699]. (ISSN: 1097-8135). http://www.lifesciencesite.com. 104