Usefulness of cytoplasmatic antigens appointing in hyperplastic cells in case of acute childhood leukaemiae
EUROMEDLAB 2003: PROCEEDINGS OF THE 15TH IFCC-FESCC EUROPEAN CONGRESS OF CLINICAL CHEMISTRY AND LABORATORY MEDICINE(2003)
Abstract
Background: The aim of this study was the evaluation of usefulness of cytoplasmatic antigens typing in difficult cases of childhood leukaemia. Materials and methods: 12 cases of leukaemia were analysed. Sur face and cytoplasmatic antigens staining with corresponding antibodies were used. IntraPrep kit was used for permabilization of cell membrane. Results: In all cases presence or absence of cytoplasmatic antigens allowed to recognise type of leukaemia, although surface antigens pattern suggested different diagnosis. Conclusions: Cytoplasmatic antigens staining is very helpful in difficulty with diagnosing childhood leukaemia.
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