KAZACHSTANIA CHRYSOLINAE AND KAZACHSTANIA BOZAE TWO NEW YEAST SPECIES OF THE GENUS KAZACHSTANIA. TRANSFER OF FOUR KAZACHSTANIA SPECIES TO GRIGOROVIA GEN. NOV. AS NEW COMBINATIONS

Four novel ascomycetous yeast strains were isolated from selected Bulgarian ecosystems. Two strains IMB190 and IMB190R were isolated from beetle guts. The strains IMBBR1 and IMBBR2 were isolated from traditional Bulgarian cereal beverage boza. Phylogenetic analysis was carried out using combined D1/D2 domains of the 26S rDNA (LSU) and internal transcribed spacer region ITS1+2 (ITS). Analysis of cut-off values of sequence identities (SI), evolutionary (ED) and physiological profile distances (PPD) of yeast species of the genus Kazachstania including new Bulgarian strains revealed that four strains represent two new yeast species. Here we present description of Kazachstania chrysolinae sp. nov. (IMB190R(inverted perpendicular)=NBIMCC 8993=CBS 12446; MycoBank MB820466) and Kazachstania bozae sp. nov. (IMBBR2(inverted perpendicular)=NBIMCC 8950=CBS12421; MycoBank MB820468) to accommodate four new Bulgarian strains. The analysis also revealed that four yeast species that previously were assigned to the genus Kazachstania represent a new yeast genus. Description of Grigorovia gen. nov. is presented (MycoBank MB820467).