Determination of Organic Acids from Mainstream Cigarette Smoke using Ultra Low Temperature Solvent Extraction Followed by Comprehensive Two-Dimensional Gas Chromatography/Time-of-Flight Mass Spectrometry

A new method for the determination of organic acids from mainstream cigarette smoke using ultra low temperature solvent extraction. comprehensive two. dimensional gas chromatography/time-of-flight mass spectrometry(GCxGC-TOF/MS) was established. The mainstream smoke was directly trapped by ethyl ether with ultra low temperature solvent extraction device, and cleaned up with liquid. liquid extraction. The concentrated extracts were further derived by N, O-bis(trimethylsily) trifluoroacetamide (BSTFA). The good separation of silanized product was achieved by the column set of DB-1 (30 m x 0. 25 mm, 1. 0 mu m) as the 1st column and DB-wax (1.5 m x 0.1 mm, 0.1 mu m) as the 2nd column with modulation period of 6 s and scan range of m/z 45-450. The results showed that linearity correlation coefficients were larger than 0. 99, and the average recoveries were between 80.17% and 107.81% with the relative standard deviations (RSD) in the range of 0.4%-12.1% (n = 5). The detection limit and the quantitation limit were 1.3-24.5 mu g/kg and 4.1-77.1 mu g/kg, respectively.