A New Methodology for Detection and Assessment of Nitric Oxide in Biological Samples

Inspired by the biochemical reaction of of L-4-Thiazolidinecarboxylic acid (thioproline, TCA) with nitric oxide in human body, a novel method was developed to quantify the NO2- in biological matrix at very high sensitivity and selectivity. The developed assay has added advantage over traditional Griess reagent and can be employed for the clinical assays of NO2- at room temperature using thioproline.