Cyanoacetamide based Barbiturates, Thiobarbiturates and their Biological Studies

Various cyanoacetamide based Knoevenagel adducts were coupled with barbituric acid / thiobarbituric acid and triethylorthoformate via a one pot three component reaction in 2-butanol availing the desired compound in excellent yields. All the synthesized compounds (2-15) were extensively characterized by H-1-NMR, C-13-NMR, Mass spectrometry and elemental analysis and were screened for antibacterial, antiurease, antioxidant, cytotoxicity and chymotrypsin inhibition studies. In case of antibacterial studies 2 was found appreciably active against the six selected strains whereas the rest of the compounds were moderately active. The urease inhibition studies revealed compound 5 and 12 as potent whereas the rest were found inactive where thiourea was used as control. Antioxidant activity results exhibited with 2 as the most active and rest of compounds showed good activity. In case of chymotrypsin inhibition studies all the synthesized compounds were found inactive with the exception of 6 which was moderately active.