Determination of Cyclopiazonic Acid in Chinese Yellow Wine by High-Performance Liquid Chromatography-Triple Quadrupole Mass Spectrometry

We developed a detection method of cyclopiazonic acid (CPA), a mycotoxin in Chinese yellow rice wine, based on high-performance liquid chromatography and triple quadrupole mass spectrometry. Under optimized conditions, the limit of detection was 50 mu g/ L. CPA recovery rates in CPA-spiked Chinese yellow rice wine were 80.1% and 85.1% at 25 mu g/ L and 1,050 mu g/ L CPA, respectively ( R > 0.9994). The relative standard deviation was 6.21-9.17%. Our developed method represents a rapid and accurate detection tool of CPA meeting minimum residue measurement requirements in Chinese yellow rice wine. The method will be widely available as a reference and be employed for Chinese official regulatory control purposes.