The Modulation of miR-195-TGF-/Smad3 Pathway on Proliferation, Apoptosis and Invasion of Glioma Cells

Glioma is one of the most common malignant tumors of the central nervous system with the high incidence. The abnormal expression of Smad3 is related to the occurrence, progression, metastasis, and drug resistance of various kinds of tumors. It was reported that the decreased expression of miR-195 was related to the onset of glioma. This study aims to explore whether miR-195 plays an important role in regulating Smad3 and influencing the cell biology of glioma. Bioinformatics analysis was performed to determine the target complementary binding site between miR-195 and Smad3. The expressions of miR-195 and Smad3 mRNA were measured in tumor tissue of patients with glioma. In vitro culture, SHG-44 cells were divided into 2 groups: miR-NC group, miR-195 mimic group. The expression of miR-195 was detected by qRT-PCR, and expression of Smad3 was measured by western blot. Apoptosis was detected by flow detection. Cell proliferation and cell invasion ability was detected by Ed U staining and Transwell experiments respectively. Our result showed that, compared with that of the carcinoma tissue, the expressions of miR-195 and Smad3 in glioma tissue were decreased significantly. Compared with normal glial cells HEB, the expression of miR-195 was decreased significantly, whereas the expression of Smad3 was increased significantly. The transfection of miR-195 mimic significantly reduced the expression Smad3 and p-Smad3, which significantly reduced the ability of cell proliferation, and increased apoptosis. In conclusion, miR-195 can target regulate proliferation, apoptosis and invasion of glioma cells through TGF-beta/Smad3 pathway.