GENUS IDENTIFICATION OF HRM SPECIES IN THE GENUS OF CARNIVOROUS FLIES

The species identification of flies is a difficult problem, especially for species of flies, which are very close to the species. Because of the difference of morphology, the traditional morphological identification method is difficult to distinguish. The gene sequence molecular identification method was developed in recent years provides a new way to solve this problem. But sequencing technologies exist time-consuming, workload is big, the higher equipment technology requirement. At present we need simple, fast, low cost technology and methods as a species identification auxiliary means. The high-resolution melting curve (high resolution melt, HRM) can meet the demand of new technology and methods. In this study, the analysis of the mitochondria] COI region and the COI]] region DNA of HRM was used to accuracy class and all kinds of flies and by using animal model to collect the common carnivorous flies in Haikou area. The results showed that the HRM test of two mt-DNA genetic regions can be achieved to distinguish the different species of flies. Even though the species can be distinguished from the species, the results showed that the results were confirmed by sequencing. According to the results of the above studies, we mapped the HRM molecular identification of 14 common species of the genus HRM in Haikou region. The species of the genus were identified, and the results were obtained. This study first proposed the idea and method of insect species identification by HRM molecular mapping. The results showed that the HRM technology has advantages of simple, fast, direct and low cost, and can be used as an important assistant tool for insect taxonomy and species identification.