pH-Dependent Specific Protein Recognition Ability of Glycopolymers

A new di-block glycopolymer, poly(gluconamidoethylmethacrylate)-b-poly[2-(diethylamino) ethyl methacrylate]-co-poly [2-(dimethylamino) ethyl methacrylate] [PGAMA-b-P (DMAEMA-co-DEAEMA), PGME] was synthesized. The chemical composition and the molecular weight of the glycopolymer were characterized by nuclear magnetic resonance(H-1 NMR) and gel permeation chromatography(GPC). The interactions of PGME and proteins were investigated by monitoring the turbidity value of the mixture solutions. PGME showed the specific biomolecular recognition ability with Concanavalin A compared with bovine serum albumin(BSA) at pH= 8. 3. When the environmental pH value changed to 7. 3, the ability of PGME specific biomolecular recognition was suppressed and PGME exhibited the behavior of non-specific binding with bovine serum albumin and Concanavalin A. Dynamic light scattering and atomic force microscopy(AFM) tests were performed to investigate the properties of the PGME-protein complex aggregations, it would be a promising to design pH-sensitive drug delivery system of saccharides.