基于寡糖代谢工程的正常和肿瘤细胞中Mucin型O-糖链的质谱定性定量比较分析
CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE（2018）
Abstract
基于寡糖代谢工程结合质谱技术(MS结合MS/MS),对4种肿瘤细胞系和1种正常细胞系中的O-糖链进行了定性和相对定量比较分析.结果表明,4种肿瘤细胞系HeLa,SMMC-7721,HepG2和MCF-7中分别检测到19,11,6和5条O-糖链;在正常肝细胞系L02中检测到10条O-糖链.在对肿瘤和正常细胞系中表达的O-糖链进行定性和相对定量比较中发现,结构组成为N1,H1N1A1和H1N1A2的3种糖链在5种细胞系中均有表达;肿瘤细胞系表达的O-糖链的种类比正常细胞多,且岩藻糖基化和唾液酸化程度均高于正常细胞组.肿瘤细胞系中特有的O-糖链主要有岩藻糖化和唾液酸化修饰的Mucin型Core2结构糖链.MS/MS分析表明,其中岩藻糖基化修饰的O-糖链结构组成为H3N3F1A2,H4N4F1A2和H5N5F1A1,唾液酸化修饰的O-糖链结构组成为H5N4A1,H4N4A2和H5N5A2.
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Key words
Mucin-type O-glycan,Tumor cell,Normal cell,Oligosaccharides metabolic engineering,Mass Spectrometry
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