High production and use of rough antigens for serological diagnosis of gambian Trypanosomiasis by ELISA and latex agglutination techniques
BULLETIN DE LA SOCIETE DE PATHOLOGIE EXOTIQUE(1996)
摘要
The extraction of rough antigens (Ag) from trypanosomes came accross a major problem getting of a high number of parasites, Moreover several technics for the extraction of rough antigens are described. Some of them are sophisticated and time consuming. Aiming the obtention of a large number of Ag for this study inoculation of trypanosomes into Cricetomys gambianus and affinity chromatography of blood allow to collect of large number of trypanosomes. This new technique leads to the extraction of 50 to 60 mg of rough Ag of trypanosomes isolated from infected Cricetomys. In order to set up a specific and sensitive serological diagnosis of gambian human trypanosomiasis, the different rough Ag were used comparatively by ELISA and Latex Agglutination tests. Confirming previous works, the results indicate id high sensitivity and a doubtful specificity for both techniques.
更多查看译文
关键词
rough antigens,serological diagnosis,cross reaction,sensitivity,specificity,Tb gambiense,Cricetomys gambianus,Cote d'Ivoire
AI 理解论文
溯源树
样例
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要