Activity and Viability of Probiotic Candidates Consisting of Lactic Acid Bacteria and Yeast Isolated from Native Poultry Gastrointestinal Tract

Live microbial cultures have a positive effect on animal digestibility by increasing the natural balance of microflora in the digestive tract. This study evaluated lactic acid bacteria (LAB) and yeast as probiotic candidates with resistance to bile salt, acid conditions, and inhibitory activities against pathogenic bacteria. The LAB and yeast were isolated from the colons of native chicken and Javanese duck, respectively. This experiment followed a factorial design. Characteristics of the isolates were evaluated as follows: antibacterial activity, antibiotic sensitivity, and viability percentage in bile salt, gastric juice and acid conditions. The result showed that both isolates had the ability to inhibit Pseudomonas aeruginosa, Staphylococcus aureus and Salmonella pullorum. The yeast B-18 isolate was resistant to streptomycin 10 mu g, penicillin 10 mu g, and erythromycin 15 mu g, while the LAB AKK-30 isolate was resistant to streptomycin 10 mu g and penicillin 10 mu g. LAB AKK-30 tends to be tolerant to bile salt, while yeast B-18 was more resistant to gastric juices and acid conditions. It concluded that both isolates are potentially useful probiotic candidates followed by microencapsulation. Further experiments should be conducted to evaluate the effectiveness of the isolates on nutrient use in poultry.