Isolation of Bovine Neutrophils by Fluorescence- and Magnetic-Activated Cell Sorting.
MYELOID-DERIVED SUPPRESSOR CELLS(2021)
摘要
Flow cytometry and magnetic bead technology enable the separation of cell populations with the highest degree of purity. Here, we describe protocols to sort bovine neutrophils from blood, the labeling and sorting, including gating strategies. We also provide advice to preserve neutrophil viability and detail a protocol to measure phagocytosis and oxidative species production.
更多查看译文
关键词
Bovine,Cell sorting,Chemiluminescence,Magnetic isolation,Neutrophils,Phagocytosis
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要